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Figure 1 | BMC Biotechnology

Figure 1

From: Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

Figure 1

Influence of the DNA extraction method on PCR efficiency. (A) Variability of PCR efficiency for different DNA isolation methods. Outlier for the CTAB procedure with proteinase K and RNase A treatment is shown as circle above the boxplot. (GS = GENESpin, CTAB.K = CTAB procedure with proteinase K and RNase A treatment). (B) The distribution of PCR efficiencies of 4 tested amplicons on different DNA extracts is presented in boxplots (efficiency data for DNA isolated with Wizard method was excluded because of high variability of results).

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