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Table 1 Evaluation of the different titration methods

From: Comparison of lentiviral vector titration methods

  RNA c/ml TU/ml pg p24/ml TU/pg TU/RNA
Cell factories      
CH-eGFP-WS 2.68 ± 0.38 × 1010 2.23 ± 1.10 × 109 9.8 ± 5.3 × 106 228 0.0832
Culture dishes      
H-eGFP 5.63 ± 0.50 × 1010 5.05 ± 4.9 × 107 4.67 ± 4.5 × 106 11 0.0009
H-eGFP-WS 3.83 ± 2.25 × 1010 2.92 ± 2.5 × 108 4.83 ± 4.70 × 106 60 0.0076
CH-eGFP-WS 2.73 ± 1.59 × 1010 1.68 ± 1.3 × 109 4.79 ± 3.45 × 106 351 0.0615
  1. The lentiviral vector CH-eGFP-WS was produced in cell factories and concentrated by centrifugation as described before [8]. Three independent RNA extractions were carried out on this vector and RNA equivalents were determined by RT-qPCR. Mean values ± standard deviation are shown. Next, three lentiviral vectors with different transfer plasmids, H-eGFP, H-eGFP-WS and CH-eGFP-WS, were produced in parallel in cell culture dishes. RNA equivalents (RNA/ml), transducing units (TU/ml) and p24 concentrations (pg p24/ml) were determined by RT-qPCR, titration and ELISA, respectively. The TU/pg and TU/RNA value indicate the specific activity of the vector constructs and correlate well with the vector backbones. The data represent the mean values ± standard deviation of three independent productions per lentiviral vector.