Overview of primer sets and lentiviral vector constructs. (A) Schematic representation of CH-eGFP-WS lentiviral vector with the corresponding amplicons of the different primer sets (LTR-gag, GFP and WPRE). Primer sequences are represented in small caps and probe sequences are in bold. (B) Schematic representation of different lentiviral vector constructs. The construct was optimized to increase transduction efficiency (cPPT and WPRE) and biosafety (SIN) as described before [9, 10, 23].