Table 2 Comparison of sample quantification methods.
From: Routes to improving the reliability of low level DNA analysis using real-time PCR
Concentration/target copies μl-1 | Number of negative reactionsNote 1 | Measured concentration | |
|---|---|---|---|
Ct of 55 included (as zero concentration)/target copies μl-1 | Ct of 55 excluded/target copies μl-1 | ||
0.5 | 10 | 0.66 | 1.48 |
1.0 | 2 | 1.68 | 1.89 |
2.0 | 3 | 2.51 | 3.02 |
5.0 | 0 | 5.28 | 5.28 |
10.0 | 0 | 10.90 | 10.90 |