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Table 1 Preparation of DNA standards. Details of the conditions used in preparation of the serial dilution DNA solutions for standard curve construction.

From: Routes to improving the reliability of low level DNA analysis using real-time PCR

Method

DNA Standard preparation condition

1

Standard plastics (1.5 ml microtubes, Alpha).

Water as diluent (Tissue culture water, Sigma, UK).

2

Standard plastics.

Stabilising solution as diluent (Stabilising component for DNA standards, Cambio, UK).

3

Standard plastics.

Carrier DNA as diluent (Herring Sperm DNA, Sigma, UK @ 10 μg/ml).

4

Low retention plastics (Non-stick hydrophobic microtubes 1.5 ml, Alpha).

Water as diluent.

5

Low retention plastics.

Stabilising solution as diluent.

6

Low retention plastics.

Carrier DNA as diluent.