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Figure 4 | BMC Biotechnology

Figure 4

From: Routes to improving the reliability of low level DNA analysis using real-time PCR

Figure 4

Calibration for low level DNA quantification. Base: All valid observations. The figure shows mean values at each concentration with a) (filled circles ) negative observations excluded; b) (open circles o) all values including negative observations taken as Ct = 55 (the means coincide at log10(C)>0.5). Regression lines are the exclusion-by-sample regression (solid line), the line through means with negative observations excluded (dashed line) and the line through means with negative observations set to Ct = 55 (dotted line).

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