pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi

BMC Biotechnology

Table 2 Inducible RFP expression in independent clones transformed with pTcINDEX-RFP.

Cell line	Uninduced	0.5 μg ml^-1tet	Ratio +tet/-tet
CL-RFP C2	0.3	71.8	239
CL-RFP A2	3.3	31.7	9.6
CL-RFP D6	0.7	25.8	36.8
CL-RFP D2	0.3	12.8	42.7
CL- RFP C5	1.1	17.5	15.9
CL-RFP B1	0.6	2.0	3.3

Each clone was induced with 0.5 μg ml^-1 tetracycline. After 5 days, cells were fixed and analysed on a FacsCalibur. Controls were identical cultures maintained in the absence of tetracycline. The data are presented as percentage of cells registering greater than 6 arbitrary fluorescence units (AFU).

ISSN: 1472-6750