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Table 2 Inducible RFP expression in independent clones transformed with pTcINDEX-RFP.

From: pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi

Cell line Uninduced 0.5 μg ml-1tet Ratio +tet/-tet
CL-RFP C2 0.3 71.8 239
CL-RFP A2 3.3 31.7 9.6
CL-RFP D6 0.7 25.8 36.8
CL-RFP D2 0.3 12.8 42.7
CL- RFP C5 1.1 17.5 15.9
CL-RFP B1 0.6 2.0 3.3
  1. Each clone was induced with 0.5 μg ml-1 tetracycline. After 5 days, cells were fixed and analysed on a FacsCalibur. Controls were identical cultures maintained in the absence of tetracycline. The data are presented as percentage of cells registering greater than 6 arbitrary fluorescence units (AFU).