Table 1 Primer sequences. PVX and epitope-specific primers, including primer sequences and amplification product characteristics for PVX6His2ACP (A), PVX6HisE212ACP (B) and PVX6HisE222ACP (C).
Name | Primer sequence 5' to 3' | Optimal annealing temp. °C | Amplified fragment size (bp) | ||
|---|---|---|---|---|---|
| Â | Â | Â | A | B | C |
PVX FOR PVX REW | CTGGGGAATCAATCACAGTG GTCGAATGCAGCGAATTTGTGC | 61.5 | 677 | 890 | 800 |
E21 FOR E21REW | GTCGACGGGCTGTGCCCATTTGATACG GTCGACACTGTTGTGGTCACACAATCC | 63 | -- | 213 | -- |
E22 FOR E22REW | GTCGACATGGATTGTGTGACCACAACA GTCGACTACTAGCCCCCCCGTGTAGAC | 64 | -- | -- | 123 |