Construction, purification, and characterization of a chimeric TH1 antagonist

BMC Biotechnology

Table 1 Balance of several processes of AnTH1 solubilization

	Total protein (mg)			% of solubilization
Process Step	Exp #1	Exp #2	Exp #3	Exp #1	Exp #2	Exp #3
Supernatant 4 M urea wash	1015.2	92.3	246	NE	NE	NE
Pellet 4 M urea wash	1045.2	378	740	NE	NE	NE
Supernatant 8 M urea solubilization	905.8	264	589	86	69.8	79.5
Pellet 8 M urea solubilization	24	16.5	23	NE	NE	NE

% of solubilization: The amount of protien in inclusion bodies in 4 M urea pellet wash solubilized with 8 M urea and present in the supernatant of 8 M urea.
(total protien (mg) supernatant 8 M urea/Total protein (mg) pellet 4 M urea) × 100.
NE: non evaluated.

ISSN: 1472-6750