From: Construction, purification, and characterization of a chimeric TH1 antagonist
Total protein (mg) | % of solubilization | |||||
---|---|---|---|---|---|---|
Process Step | Exp #1 | Exp #2 | Exp #3 | Exp #1 | Exp #2 | Exp #3 |
Supernatant 4 M urea wash | 1015.2 | 92.3 | 246 | NE | NE | NE |
Pellet 4 M urea wash | 1045.2 | 378 | 740 | NE | NE | NE |
Supernatant 8 M urea solubilization | 905.8 | 264 | 589 | 86 | 69.8 | 79.5 |
Pellet 8 M urea solubilization | 24 | 16.5 | 23 | NE | NE | NE |