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Table 1 Balance of several processes of AnTH1 solubilization

From: Construction, purification, and characterization of a chimeric TH1 antagonist

  Total protein (mg) % of solubilization
Process Step Exp #1 Exp #2 Exp #3 Exp #1 Exp #2 Exp #3
Supernatant 4 M urea wash 1015.2 92.3 246 NE NE NE
Pellet 4 M urea wash 1045.2 378 740 NE NE NE
Supernatant 8 M urea solubilization 905.8 264 589 86 69.8 79.5
Pellet 8 M urea solubilization 24 16.5 23 NE NE NE
  1. % of solubilization: The amount of protien in inclusion bodies in 4 M urea pellet wash solubilized with 8 M urea and present in the supernatant of 8 M urea.
  2. (total protien (mg) supernatant 8 M urea/Total protein (mg) pellet 4 M urea) × 100.
  3. NE: non evaluated.