Figure 4
From: Construction, purification, and characterization of a chimeric TH1 antagonist
Electrophoretic mobility of recombinant chimeric protein in reduced and non-reduced conditions. Panel A: The antagonist was identified in a Western blot using a rabbit anti-IL-2 polyclonal antibody. The analyzed TH1 antagonist preparations were those obtained from the 8 M urea extraction, (lanes a and b), gel filtration fraction in 4 M urea (lanes c and d) and after dialysis against PBS (lanes e and f). Panel B: Western blot to identify the TH1 antagonist after dialysis against PBS (lane g) and IL-2 (lane h) in reduced conditions using the anti-IL-2 antibody.