Figure 3
From: Construction, purification, and characterization of a chimeric TH1 antagonist
Binding assay of rhu 125I-IFN-γ labeled to recombinant chimeric protein using dot blot analysis. 1 μl aliquots of the eluted fractions from the gel filtration chromatography (rows 1, 2, 3, 4, 5, 6) were applied to nitrocellulose membrane strips. The strips were incubated with IFN-γ labeled with 125I (35 μCi/μg) in the absence (lane a) and in the presence (lane b) of 100 fold excess of human IFN-γ.