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Figure 1 | BMC Biotechnology

Figure 1

From: Multi-substrate chromosome preparations for high throughput comparative FISH

Figure 1

(a-i) Multicolour FISH applied to triple substrate chromosome preparations. (a-c) Retrotransposon-based probes KK2.5 (yellow fluorescence), KK3.4 (green fluorescence) and KK4.24 (red fluorescence) hybridising to somatic metaphase chromosomes of (a) Avena agadiriana (2n = 4x = 28; genomes AABB), (b) A. murphyi (2n = 4x = 28; genomes AACC) and (c) A. sterilis (2n = 6x = 42; genomes AACCDD). (d-f) BAC clone-based probes ABR1-41-A8 (green fluorescence) and ABR1-47-F4 (purple pseudocolour), ABR1-59-F9 (red fluorescence) and 25S rDNA-based probe (yellow fluorescence) hybridised to somatic metaphase chromosomes of (d) Brachypodium distachyon (2n = 2x = 10), (e) unidentified, presumably diploid species of Brachypodium ABR114 (2n = 2x = 20) and (f) an interspecific hybrid ABR113 (2n = 4x = 30) containing the two genomes shown in (d, e). (g-i) BAC clones ABR1-41-E10 (green fluorescence) and ABR5-1-H3 (red fluorescence) hybridising to somatic metaphase chromosomes of (g) B. distachyon, (h) ABR114 (2n = 2x = 20) and (i) ABR113 (2n = 4x = 30). (j) Diagram of a triple substrate chromosome preparation. Scale bar: (a-c) 10 μm, (d-i) 5 μm.

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