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Figure 5 | BMC Biotechnology

Figure 5

From: A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects

Figure 5

Presence of ButaIT/GNA fusion protein in gut and haemolymph of L. oleracea larvae following feeding. Samples were taken from third stadium L. oleracea larvae fed for 12 days on control diet (C), or diet containing ButaIT/GNA (F; 4.5 mg/5 g diet). Proteins were resolved by 15 % acrylamide SDS-PAGE gel, electroblotted onto nitrocellulose, and probed with anti-GNA antibodies. Loading is as follows: lanes 1, 3, 5 and 11 are control diet, gut tissue, gut contents and haemolymph respectively; lanes 2, 4, 6, 7 and 12 are ButaIT/GNA diet, gut, gut contents and haemolymph; lane 8 is a GNA standard (10 ng); lanes 9 & 10 are ButaIT/GNA standards of 25 and 50 ng, respectively. Loading, as estimated by Bradford assay, was 40 μg total protein for all gut and haemolymph samples.

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BMC Biotechnology

ISSN: 1472-6750

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