Figure 4
NIH3T3 cells transfected with the GFP-TN-C promoter construct were plated onto the different preparations at the same density, and at 24 hours after plating, they were fixed and stained with DAPI and Texas Red-C2-maleimide. Cells in 50 fields were imaged and analyzed on each sample using automated digital microscopy and image analysis software. A) Average relative GFP intensity is reported as a function of surface preparation. All data are statistically different from one another at p < 0.05. B) Average cell area is reported as a function of surface preparation. Error bars reflect the standard deviation of a minimum of four samples derived from at least two replicate experiments.