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Table 1 donor vectors constructs

From: Modification of the Creator recombination system for proteomics applications – improved expression by addition of splice sites

Vector ID Vector Name Potential Splicing Events Description
V1 pDNR Dual Splice for 3' tags Designed for Fusions at both N- and C- termini (Clontech)
V7 pDNR-MCS Splice for 3' tags Alternate MCS that adds AscI and PacI sites to the MCS of V1
V37 pDNR MCS SA Splice for 5' and 3' tags A splice acceptor introduced into V7 before the AscI site
V308 pDNR MCS lacZ alpha Splice for 3' tags LacZ alpha inserted between AscI and PacI sites of V7
V309 pDNR MCS SA lacZ alpha Splice for 5' and 3' tags LacZ alpha inserted between AscI and PacI sites of V37
V624 pDNR MCS BE Splice for 3' tags Alternate MCS with BamHI, SalI, SmaI, HindIII, EcoRI sites
V677 pDNR MCS BE lacZ alpha Splice for 3' tags LacZ alpha inserted between SalI and HindII sites of V624
V678 pDNR MCS SA ES Splice for 5' and 3' tags Alternate MCS Splice Acceptor with AscI, EcoRI, NotI, BamHI, SalI, PacI sites.
V795 pDNR MCS ES Splice for 3' tags Alternate MCS with AscI, EcoRI, NotI, BamHI, SalI, PacI sites
V954 pDNR MCS SA no SA CmR Splice for 5' and 3' tags Silent mutations introduced in the CmR ORF of V309 to remove potential splice acceptor
  1. *Abbreviations used: SA – splice acceptor, MCS – multiple cloning site, CmR – chloramphenicol resistance, BE – BamHI/EcoRI, ES – EcoRI/SalI