TbPFR2 silencing by targeting its UTRs all together. WT cells were transfected with GFP dsRNA (A), CDS dsRNA (B) or UTRs MIX dsRNAs (C). At 14 h post-transfection, cells were treated for immunofluorescence using the L8C4 anti-TbPFR2 antibody (green) and counterstained with DAPI (blue). Cells of interest are bi-nucleated/bi-flagellated. Transfection of GFP dsRNA did not produce any specific phenotype. Both the CDS dsRNA and the UTRs MIX dsRNAs successfully silenced TbPFR2. Scale bar: 10 μm. See text for details.