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Table 2 Construction of human diabodies. Primer combinations and primer sequences

From: Generation of human antibody fragments against Streptococcus mutansusing a phage display chain shuffling approach

Template

VL amplification

VH amplification

pHenIX human scFv B10

Vκ1 ApaL1

Jκ1 Not1

VH4 Sfi1/Nco1

JH3 for Sal1

pHenIX human scFv H6

Vκ1 ApaL1

Jκ1 Not1

VH6 Sfi1/Nco1

JH2 for Sal1

pHenIX human scFv D12

Vκ1 ApaL1

Jκ1 Not1

VH4 Sfi1/Nco1

JH2 for Sal1

pHenIX scFv mGuy13

mGuy13 ApaL1

mGuy13 Not1

VH4 Sfi1/Nco1

mGuy13 Sal1

  1. Vκ1 ApaLI: 5'-TGAGCACACAGTGCACTCGACATCCAGATGACCCAGTCTCC-3'
  2. Jκ1 Not1: 5'-GAGTCATTCTCGACTTGCGGCCGCACGTTTGATC/TTCCAC/GCTTGGTCCC-3'
  3. VH4 Sfi1/Nco1: 5'-GTCCTCGCAACTGCGGCCCAGCCGGCCATGGCCCAGGTGCAGCTGCA/ GGAGTCGGG-3'
  4. VH6 Sfi1/Nco1: 5'-GTCCTCGCAACTGCGGCCCAGCCGGCCATGGCCCAGGTACAGCTGCA/ GCAGTCAGG-3'
  5. JH3 Sal1: 5'-GAGTCATTCTCGTGTCGACACGGTGACCATTGTCCC-3'
  6. JH2 Sal1: 5'-GAGTCATTCTCGTGTCGACACAGTGACCAGGGTGCC-3'
  7. mGuy13 ApaL1I:5'-TGAGCACACAGTGCACTCGACATCGAGCTCACTCAGTCTCC-3'
  8. mGuy13 Not1: 5'-TTTTCCTTTTGCGGCCGCCCGTTTTATTTCCAACTTTGT-3'
  9. mGuy13 Sal1: 5'-GAGTCATTCTCGTGTCGACACGGTGACCGTGGTGCCTTGGCCCCAGTAGTCAAAGTAGGT-3'
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