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Table 2 Description of data sets 1 and 2 used for comparing regression coefficients. Data sets 1 and 2 were produced based on measuring the instrument response (Ct values) in relation to a range of standards of known copy number. The copy numbers of these standards are displayed in the first column, with the log to the base 10 of these copy numbers displayed in the second column. The Y1 column shows the Ct values corresponding to data set 1 from the first RT-QPCR platform, whilst the Y2 columns shows the Ct values corresponding to data set 2 from the second RT-QPCR platform.

From: Standardisation of data from real-time quantitative PCR methods – evaluation of outliers and comparison of calibration curves

DNA copy nu

Log (DNA copy nu)

Y1

Y2

31628

4.5

18.79

20.93

10000

4

20.47

22.74

3162

3.5

22.31

24.56

1000

3

25.44

27.31

316

2.5

28.74

30.11

100

2

32.24

33.38

32

1.5

34.82

36.37