Table 1 Strains and plasmids used in this work

S. gordonii

V288

Recipient in transformation

[35]

GP201

ΩTn5253, Sm^R, Cm^R, Tc^R

[13]

GP204

str-204, Sm^R

[13]

GP214

pKT random in V288, Cm^R

This work

GP215

pKT random in V288, Cm^R

This work

GP216

pKT random in V288, Cm^R

This work

GP231

emm6, Er^R, Sm^R

[21]

GP1241

ΩTn5253 (ΔtetM::pSMB139) Er^R, Sm^R, Cm^R, Tc^S

This work

GP1253

emm6.1::TTFC

[28]

E. faecalis

OG1SS

Recipient in conjugation, Sm^R, Sp^R

[20]

GP431

ΩTn916, Er^R, Tc^S (Δ tetM::pSMB156)

This work

B. subtilis

GP800.2

ΩTn916, Tc^R

[12]

GP847

ΩTn916, Er^R, Tc^S (Δ tetM::pSMB156)

This work

GP848

ΩTn916, Er^R, Tc^S (Δ tetM::pSMB288)

This work

Plasmid

Description

Source/reference

pKT

Ap^R, Cm^R

[25]

pBluescript

Ap^R

Stratagene

pVMB5

pBluescript::PP-cat, Ap^R, Cm^R

This work

pVMB20

pBluescript::emm6.1::ermC, Ap^R, Er^R

[21]

pVA891

Cm^R, Er^R

[27]

pSMB47

pVA891 derivative containing DNA sequences from Tn5253, Cm^R, Er^R

[12]

pSMB89

pVA891::emm6, Cm^R, Er^R

This work

pSMB128

pVA891::PP-emm6, Cm^R, Er^R

This work

pSMB139

pSMB47::PP-emm6, Cm^R, Er^R

This work

pSMB148

pSMB47::PP-emm6/55, Cm^R, Er^R

This work

pSMB156

pSMB47::PP-emm6/55::ova, Cm^R, Er^R

This work