Figure 5From: Genetic and spectrally distinct in vivoimaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent proteinWidespread RFP expression in adult organs. Panels of bright field and corresponding dark field epifluorescent images of organs taken from a 4 week old CAG::mRFP1 Tg/+ mouse and a non-transgenic littermate. Peritoneum (A), heart (B), lung (C), eye (D), brain (E), liver (F), pancreas (G), spleen (H) and kidney (I). In addition to the fluorescence observed under epifluorescent illumination, RFP expressing tissues exhibit a pink color under bright field illumination. This is particularly evident in panels A, B, C, E and G. This allows for genotyping of newborn pups (by virtue of their pink color) in the absence of fluorescence illumination.Back to article page