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Table 2 Variation attributable to day, plasmid copy number, PCR reaction mix and platform following an ANOVA test using threshold cycle number (Ct) as the variable factor.

From: An inter-platform repeatability study investigating real-time amplification of plasmid DNA

  Univariate Tests of Significance for Ct value. Sigma-restricted parameterisation. Effective hypothesis decomposition.
Effect SS DF MS F P
Day 96.0 2 48.0 221.4 <0.00001
Plasmid Copies 12919.2 6 2153.2 9932.0 <0.00001
PCR Reaction Mix 75.0 1 75.0 346.2 <0.00001
Platform 700.5 2 350.2 1615.5 <0.00001
Day with Plasmid Copies 53.2 12 4.4 20.5 <0.00001
Day with PCR Reaction Mix 1.9 2 0.9 4.3 0.01442
Plasmid Copies with PCR Reaction Mix 8.5 6 1.4 6.5 <0.00001
Day with Platform 130.8 4 32.7 150.8 <0.00001
Plasmid Copies with Platform 51.0 12 4.2 19.6 <0.00001
PCR Reaction Mix with Platform 1.7 2 0.9 4.0 0.01901
Day with Plasmid Copies with PCR Reaction Mix 14.6 12 1.2 5.6 <0.00001
Day with Plasmid copies with Platform 18.3 24 0.8 3.5 <0.00001
Day with PCR Reaction Mix with Platform 49.6 4 12.4 57.1 <0.00001
Plasmid copies with PCR Reaction Mix with Platform 4.5 12 0.4 1.7 0.06337
Day with Plasmid Copies with PCR Reaction Mix with Platform 11.8 24 0.5 2.3 0.00091
Error 54.4 251 0.2   
  1. (Where SS = sum of squares; DF = degrees of freedom; MS = Mean Square; F = ratio of variances; p = probability of obtaining a specific result, given by the null hypothesis).