Figure 3
From: A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system
Demonstration of dual drug control. In Cos7 and HeLa cells, optimized ratios for the parental Ariad dimerizer System (A), tTA TET-OFF System (pRetro-OFF) (B), the merged Dual Drug Control (DDC) system (C), and the Tet-SEAP reporter in isolation (D) are evaluated for activity in the presence or absence of dimerizer and/or tetracycline. Values shown reflect luminometer readings of SEAP reporters. Blue boxes reflect values obtained in Cos7 cells; yellow boxes, in HeLa cells. Standard deviation is indicated by error bars. Each experiment was repeated at least three times.