Figure 2

Polyclonal phage ELISA. Polyclonal phage ELISAs showing the increase in specific signal with successive rounds of panning. Phages were selected by panning on immobilised proteins, haptens and viruses. Pools released from the affinity matrix after each round of selection were amplified, purified by precipitation and tested for binding to the homologous target and several heterologous antigens: BSA = bovine serum albumin, MP = 2% milk powder in PBS, MBP = maltose binding protein, F-BSA = fluorescein-conjugated bovine serum albumin, N-BSA = 4-hydroxy-3-iodo-5-nitrophenylacetic acid conjugated to bovine serum albumin, AHSV = purified African horsesickness virus particles, BTV = purified bluetongue virus particles, CMV = purified cucumber mosaic virus particles, CYT = cytochrome C, THY = porcine thyroglobulin, CHY = chymotrypsinogen, KLH = keyhole limpet haemocyanin, TEL = turkey egg lysozyme. The homologous antigens in each case were: A) F-BSA, B) N-BSA, C) KLH, D) TEL, E) CYT, F) THY, G) CMV, H) AHSV and I) BTV. Figures on the X axis indicate the panning round of which the output was tested for polyclonal binding. The figure 0 refers to the unpanned library.