Figure 1From: The defH9-iaaM auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberrySouthern blot analysis of strawberry and raspberry plants transgenic for DefH9-iaaM. Genomic DNA digested with HindIII from: F. vesca control plants (panel a, lane1), and transgenic lines 1 and 2 (panel a, lanes 2, 3 respectively); F. x ananassa control plants (panel b, lane1) and transgenic line 1 (panel b, lane 2); R. idaeus control plants (panel c, lane 1) and transgenic line 1 (panel c, lane 2). The blots were probed with a 589 bp DNA fragment of the iaaM coding region that does not contain the HindIII site present in the iaaM coding region. The probe was obtained by PCR using the following primers 5'AAACAAGCTTCCCACCACCATCCAG3' and 5'CATGCTCTTTTCACCCGTATTAG3'.Back to article page