Table 2 N-Terminal fusion expression comparison
| Â | N-TERMINAL FUSION | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Protein (domain) | H6 | H10 | H10-GFP | H10-GST | H10-Trx | H10-MBP | ||||||
| Â | T | S | T | S | T | S | T | S | T | S | T | S |
CASP2 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.20 | 0.20 | 2.50 | 2.11 |
CCND2 | 21.85 | 0.00 | 12.36 | 5.81 | 6.14 | 0.02 | 1.20 | 0.00 | 12.50 | 4.35 | 8.12 | 3.06 |
CD44 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | nc | nc | 0.00 | 0.00 | 0.00 | 0.00 |
CDK2 | 14.81 | 1.84 | 1.03 | 0.07 | nc | nc | 4.88 | 2.17 | 2.00 | 1.54 | 25.00 | 25.00 |
CDK4 | 8.78 | 0.71 | 1.47 | 1.37 | nc | nc | 1.32 | 0.00 | nc | nc | 2.79 | 0.00 |
CDKN1B | 7.44 | 0.17 | 0.85 | 0.31 | 1.63 | 0.57 | 12.00 | 4.30 | 4.00 | 1.69 | 8.00 | 5.19 |
CDKN2A | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.40 | 0.32 | nc | nc | 3.65 | 0.00 |
Efna1 | 0.00 | 0.03 | 1.50 | 1.33 | 7.47 | 0.22 | 2.29 | 0.06 | nc | nc | 5.73 | 3.02 |
Efna1 (EC) | 24.74 | 0.05 | 5.00 | 4.81 | 50.00 | 1.71 | 28.00 | 0.07 | 60.00 | 4.10 | 11.93 | 4.93 |
Efnb2 (EC1) | 3.58 | 0.00 | 11.53 | 1.18 | 10.38 | 0.86 | 6.43 | 1.30 | 44.70 | 6.82 | 22.67 | 20.00 |
Efnb2 (EC2) | 2.07 | 0.04 | 3.00 | 2.79 | 50.00 | 6.50 | 55.00 | 0.00 | nc | nc | 17.00 | 16.00 |
EGFR (TK) | 0.00 | 0.00 | 0.21 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Epha2 (LB) | nc | nc | nc | nc | 50.00 | 0.43 | 2.83 | 0.05 | 4.92 | 1.99 | 14.57 | 3.78 |
Ephb2 (LB) | 40.00 | 0.00 | 0.66 | 0.08 | 4.93 | 0.14 | 10.00 | 0.03 | 29.39 | 2.53 | 20.00 | 2.11 |
Ephb2 (SAM) | 0.00 | 0.00 | nc | nc | 50.00 | 5.63 | 35.00 | 11.47 | 10.06 | 1.34 | 0.00 | 0.00 |
Ephb2 (TK) | 0.00 | 0.00 | 65.84 | 15.00 | 20.00 | 0.35 | 15.00 | 0.14 | 50.00 | 14.23 | 20.00 | 4.15 |
Fli1 | 3.82 | 0.05 | 0.89 | 0.08 | 2.00 | 0.00 | 1.50 | 0.00 | 12.00 | 5.14 | 58.00 | 6.50 |
FOS | 0.25 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.49 | 0.08 | 0.27 | 0.00 |
GATA2 | 0.33 | 0.00 | 0.19 | 0.07 | 2.50 | 0.04 | 0.00 | 0.00 | 5.00 | 3.47 | 0.00 | 0.00 |
GFP | 60.00 | 60.00 | 25.00 | 25.00 | 25.00 | 1.33 | 22.08 | 20.00 | 25.00 | 25.00 | 10.00 | 9.83 |
GRB2 | 1.75 | 0.04 | 6.00 | 3.88 | 25.00 | 12.82 | 2.77 | 0.84 | 13.00 | 11.96 | 18.00 | 16.00 |
HRAS | 30.10 | 0.34 | 6.40 | 5.59 | 6.16 | 0.17 | 7.37 | 0.54 | 26.96 | 25.00 | 8.40 | 7.69 |
JUN | 40.00 | 0.00 | 5.84 | 1.09 | 2.08 | 0.00 | 1.50 | 0.00 | 5.70 | 0.41 | 30.00 | 0.22 |
MAD | 32.77 | 0.15 | 3.50 | 1.78 | 20.66 | 0.37 | 15.04 | 0.13 | 9.21 | 4.74 | 4.00 | 4.10 |
MAX | nc | nc | 9.43 | 1.09 | 4.44 | 1.18 | 0.00 | 0.03 | 2.05 | 2.01 | 3.00 | 2.71 |
Mdm2 | 0.00 | 0.00 | 1.20 | 0.91 | 0.00 | 0.00 | 0.00 | 0.00 | 10.00 | 5.57 | 3.60 | 2.65 |
Mdm2 (p53-bd) | 1.62 | 0.36 | 4.75 | 4.70 | 20.84 | 3.20 | 20.00 | 0.22 | 9.54 | 4.72 | 12.00 | 12.00 |
MMP1 | 2.56 | 0.00 | 0.36 | 0.10 | 11.44 | 0.00 | 39.63 | 0.04 | 0.32 | 0.32 | 30.00 | 0.48 |
RAF1 (Ras-bd) | 15.48 | 15.00 | 20.00 | 20.00 | 26.92 | 0.00 | 20.00 | 19.82 | 25.00 | 25.00 | 40.00 | 25.00 |
Trp53 | 0.85 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
AVERAGE | 11.36 | 0.81 | 7.28 | 3.27 | 10.53 | 1.01 | 9.02 | 1.37 | 15.90 | 6.02 | 14.87 | 5.81 |