Figure 1

Regression lines displaying accuracy and sensitivity of quantitative genotyping of SNPs. The regression lines were obtained by analysing ten SNPs in a series of mixed samples with varying amounts of DNA from two individuals of different genotype. The signal intensity ratios from minisequencing using immobilised primers (Method I, black diamonds) and "tag-array" minisequencing (Method II, grey squares) are plotted as a function of the known allelic ratios in the mixed samples. The SNP names are given in the panels. The signal intensity ratios are mean values of five replicate reactions. The signal intensity ratios obtained in homozygous samples (allele ratios zero) are indicated as black diamonds and grey squares on the left vertical axis of each panel.