In-frame <EYOR> transposition events in the voltage sensitive integral membrane motor protein, Prestin. Visual screening of transiently transfected HEK-293 cells revealed truncated Prestin-YFP fusion proteins generated by in-frame <EYOR> transpositions (A) (scale bar = 20 μm). Alternate digestion and re-ligation of an in-frame clone with either Srf I or Asc I produces identical full-length YFP- (B) and CFP-fusion proteins (C). In-frame <EYOR> insertions in Prestin are identified by the first of 3 target amino acids duplicated during transposition (D). Redundant insertions are indicated by the number of recovered clones (e.g. 3x).