Figure 2

RT-PCR analysis of mRNA transcribed from the tricistronic vectors in stably transduced melanoma cells. 2A : Localization of the different primers. Figure 2B: cDNA obtained after reverse transcription was amplified using primers 1 and 5 (lanes 1 to 4) or primers 4 and 10 (lanes 5 to 8) as described in Methods. Lanes 1 and 5: RT-PCR performed on mRNA isolated from TFG^FGF-2ZEO transduced cells (1819 and 1640 bp amplified respectively). Lanes 2 and 6: RT-PCR performed on mRNA isolated from TFG^EMCVZEO transduced cells (1819 and 1640 bp amplified respectively). Lanes 3 and 7: RT-PCR performed on mRNA isolated from TFG^HTLV-1ZEO transduced cells (1510 and 1640 bp amplified respectively). Lanes 4 and 8: RT-PCR performed on mRNA isolated from mock retroviral vector transduced cells (negative controls).