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Table 1 Chimeric primers for RT and primers for PCR.

From: A new reverse transcription-polymerase chain reaction method for accurate quantification

Primers Sequences
For RT  
Rat VHL  
Short 5'-CGGCTCGTGACTGCTATCTTC AATGTGATGTTGGCAAAAAT-3'
Long 5'-CGGCTCGTGACTGCTATCTTC(N)nAATGTGATGTTGGCAAAAAT-3'
Rat Collagen I  
Short 5'-CGGCTCGTGACTGCTATCTTC CCTTGGCACCATCGTTGC-3'
Long 5'-CGGCTCGTGACTGCTATCTTC(N)nCCTTGGCACCATCGTTGC-3'
For PCR  
Rat VHL  
Upper 5'-GGGACCCATGATGGACTTCT-3'
Lower 5'-CGGCTCGTGACTGCTATCTTC-3'
Rat Collagen I  
Upper 5'-CCCTGGTGAACGTGGTGTAC-3'
Lower The same as the VHL lower primer
  1. Gene-specific sequences: letters in italic; a universal sequence: bold letters; the linkers (N)n: either (A)10, (C)10, (G)10, (AT)5, or (A)5(G)5(A)5.