From: A new reverse transcription-polymerase chain reaction method for accurate quantification
Primers | Sequences |
---|---|
For RT | Â |
Rat VHL | Â |
Short | 5'-CGGCTCGTGACTGCTATCTTC AATGTGATGTTGGCAAAAAT-3' |
Long | 5'-CGGCTCGTGACTGCTATCTTC(N)n AATGTGATGTTGGCAAAAAT-3' |
Rat Collagen I | Â |
Short | 5'-CGGCTCGTGACTGCTATCTTC CCTTGGCACCATCGTTGC-3' |
Long | 5'-CGGCTCGTGACTGCTATCTTC(N)n CCTTGGCACCATCGTTGC-3' |
For PCR | Â |
Rat VHL | Â |
Upper | 5'-GGGACCCATGATGGACTTCT-3' |
Lower | 5'-CGGCTCGTGACTGCTATCTTC-3' |
Rat Collagen I | Â |
Upper | 5'-CCCTGGTGAACGTGGTGTAC-3' |
Lower | The same as the VHL lower primer |