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Table 3 Permeation of E. coli cells through independent repeated cycles (1°, 2°, 3°) of slow freezing/ thawing/ sonication (FTS), followed by two three-phase partitioning (TPP) extractions, and elution throughout hydrophobic HIC chromatography column. The specific growth rate constant (μ) of 0.99 h-1 for culture A, 1.05 h-1 for cultures B, C, D, E and 1.10 h -1 for cultures F, G, H.

From: Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp uv ) from Escherichia coli

     Permeation (FTS)    
Sample (-75°C) 1° FTS 2° FTS 3° FTS Pool TPP
  gfp uv μg/mL BSA mg/mL gfp uv / BSA μg/mg gfp uv μg/mL BSA mg/mL gfp uv / BSA μg/mg gfp uv μg/mL BSA mg/mL gfp uv / BSA μg/mg gfp uv μg/mL BSA mg/mL gfp uv / BSA μg/mg gfp uv μg/mL BSA mg/mL gfp uv / BSA μg/mg gfp uv μg/mL BSA mg/mL gfp uv / BSA mg/mL
A1 69.85 0.81 86.25 57.83 2.80 20.62 91.78 3.89 23.61 21.73 0.61 35.87     44.49 1.10 40.32
A2 15.23 1.95 9.24     12.04 5.06 2.38 5.78 5.14 1.12     17.83 0.48 37.52
A3 2.95 0.20 14.81                
(Pool) 44.79 1.75 25.57 57.85 2.80 20.62 55.18 4.18 13.19 21.58 1.66 13.00 41.14 2.15 19.11 23.97 1.64 14.59
B 6.81 0.35 19.72 7.75 1.77 4.36 4.93 0.79 6.27 3.28 0.48 6.84 13.77 1.54 8.93 18.40 1.34 13.73
C 5.39 0.59 9.09 13.73 2.00 6.86 2.77 0.57 4.88 3.37 0.59 5.75 13.46 1.33 10.13 4.28 0.55 7.78
D 5.39 0.47 11.37 7.02 0.92 7.65 2.82 0.59 4.76 1.38 0.20 6.93 12.44 0.62 20.10 2.16 0.40 5.40
E 7.10 0.38 18.70 16.54 1.75 9.43 3.71 0.57 6.54 1.26 0.14 9.13 13.40 0.96 13.97 2.14 0.24 8.81
F 2.52 0.21 11.93 17.62 2.24 7.87 4.50 1.18 3.82 2.57 0.82 3.12 5.35 1.19 4.50 2.53 0.56 4.50
G 1.54 0.47 3.28 20.39 1.45 14.02 4.47 0.74 6.04 3.11 0.51 6.14 5.96 0.91 6.52 20.32 1.28 15.89
H 4.06 0.23 17.43 23.95 4.02 5.96 4.70 1.04 4.50 4.27 0.45 9.56 7.03 0.57 12.26 16.53 0.98 16.79