BMC Biotechnology

Table 3 Permeation of E. coli cells through independent repeated cycles (1°, 2°, 3°) of slow freezing/ thawing/ sonication (FTS), followed by two three-phase partitioning (TPP) extractions, and elution throughout hydrophobic HIC chromatography column. The specific growth rate constant (μ) of 0.99 h-1 for culture A, 1.05 h-1 for cultures B, C, D, E and 1.10 h -1 for cultures F, G, H.

From: Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp_uv) from Escherichia coli

				Permeation (FTS)
Sample	(-75°C)			1° FTS			2° FTS			3° FTS			Pool			TPP
	gfp_uv μg/mL	BSA mg/mL	gfp_uv/ BSA μg/mg	gfp_uv μg/mL	BSA mg/mL	gfp_uv/ BSA μg/mg	gfp_uv μg/mL	BSA mg/mL	gfp_uv/ BSA μg/mg	gfp_uv μg/mL	BSA mg/mL	gfp_uv/ BSA μg/mg	gfp_uv μg/mL	BSA mg/mL	gfp_uv/ BSA μg/mg	gfp_uv μg/mL	BSA mg/mL	gfp_uv/ BSA mg/mL
A1	69.85	0.81	86.25	57.83	2.80	20.62	91.78	3.89	23.61	21.73	0.61	35.87				44.49	1.10	40.32
A2	15.23	1.95	9.24				12.04	5.06	2.38	5.78	5.14	1.12				17.83	0.48	37.52
A3	2.95	0.20	14.81
(Pool)	44.79	1.75	25.57	57.85	2.80	20.62	55.18	4.18	13.19	21.58	1.66	13.00	41.14	2.15	19.11	23.97	1.64	14.59
B	6.81	0.35	19.72	7.75	1.77	4.36	4.93	0.79	6.27	3.28	0.48	6.84	13.77	1.54	8.93	18.40	1.34	13.73
C	5.39	0.59	9.09	13.73	2.00	6.86	2.77	0.57	4.88	3.37	0.59	5.75	13.46	1.33	10.13	4.28	0.55	7.78
D	5.39	0.47	11.37	7.02	0.92	7.65	2.82	0.59	4.76	1.38	0.20	6.93	12.44	0.62	20.10	2.16	0.40	5.40
E	7.10	0.38	18.70	16.54	1.75	9.43	3.71	0.57	6.54	1.26	0.14	9.13	13.40	0.96	13.97	2.14	0.24	8.81
F	2.52	0.21	11.93	17.62	2.24	7.87	4.50	1.18	3.82	2.57	0.82	3.12	5.35	1.19	4.50	2.53	0.56	4.50
G	1.54	0.47	3.28	20.39	1.45	14.02	4.47	0.74	6.04	3.11	0.51	6.14	5.96	0.91	6.52	20.32	1.28	15.89
H	4.06	0.23	17.43	23.95	4.02	5.96	4.70	1.04	4.50	4.27	0.45	9.56	7.03	0.57	12.26	16.53	0.98	16.79

Back to article page

ISSN: 1472-6750

Contact us

General enquiries: journalsubmissions@springernature.com