FISH analysis for transgene localization. The transgene was located to chromosome 15, region q25–q28, in TG pig #152 (F1 generation) by FISH. A 3.1 kb EcoR I/Sal I DNA fragment from the plasmid vector region of the pSEAP2-Control (# 2308–260) was used as a probe. The standard FISH experiment was performed by SeeDNA Biotech Inc. (Windsor, Ontario, Canada). The detailed localization was further determined based on the summary from ten photos. DAPI (4', 6'-Diamidino-2-phenylindole Dihydrochloride) is a DNA-specific fluorescent dye. FITC: fluorescein isothiocyanate.