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Table 3 Real-time PCR estimates of copy number for TSWV-N and nptII transgenes and possible rearrangements. For each line and each gene the estimate of copy number was derived from values in Table 2, as the range of integers that are included in the 95% confidence interval around the ratio r line /r1. The column on the right indicates evidence for rearrangements in the T-DNA.

From: Estimating the number of integrations in transformed plants by quantitative real-time PCR

Line TSWV-N nptII rearranged
111–6 1 1 No
99–1 1 1 No
1–2 c 1 2 Yes
110–1 2 2 * No
46–1 2 1 Yes
80–1 2 2 * No
127–1 2 4 Yes
133–1 3 3 No
1–1 3 2 Yes
30–4 3–4 3 ?
113–12 3–4 4 ?
20–2 7–10 9–10 ?
197–1 7–11 8–10 ?
118–2 0 5 Yes
Hom.110–1 2 § 2 § No
  1. * As no integer in the 95% confidence interval was found, the nearest integer to r line / r 1 was chosen. §For the homozigous 110–1 line, containing the double amount of transgene, the copy number was calculated as [(r line / r 1 )/2].