Table 3 Real-time PCR estimates of copy number for TSWV-N and nptII transgenes and possible rearrangements. For each line and each gene the estimate of copy number was derived from values in Table 2, as the range of integers that are included in the 95% confidence interval around the ratio r line /r1. The column on the right indicates evidence for rearrangements in the T-DNA.
From: Estimating the number of integrations in transformed plants by quantitative real-time PCR
Line | TSWV-N | nptII | rearranged |
|---|---|---|---|
111–6 | 1 | 1 | No |
99–1 | 1 | 1 | No |
1–2 c | 1 | 2 | Yes |
110–1 | 2 | 2 * | No |
46–1 | 2 | 1 | Yes |
80–1 | 2 | 2 * | No |
127–1 | 2 | 4 | Yes |
133–1 | 3 | 3 | No |
1–1 | 3 | 2 | Yes |
30–4 | 3–4 | 3 | ? |
113–12 | 3–4 | 4 | ? |
20–2 | 7–10 | 9–10 | ? |
197–1 | 7–11 | 8–10 | ? |
118–2 | 0 | 5 | Yes |
Hom.110–1 | 2 § | 2 § | No |