Table 1 Sequences of primer pairs used in multiplex reactions and expected sizes of PCR products
From: Large scale sex typing of ostriches using DNA extracted from feathers
Forward primers | Reverse primers | Length of PCR product |
|---|---|---|
SS1 5'-TCTACACCTAAGGAGTCCCATATT-3' | SS2 5'-GGTCTACACCTGTTGAAAATCATT-3' | 648 bp |
OSM5f 5'-GTGGATCAGTTCAATCCTTGC-3' | OSM5r 5'-GCCCAAGAAAATGATGGAGA-3' | 232 bp |
OSM7f 5'-AGCATACACATGCAGACCCC-3' | OSM7r 5'-TGTTTCCTGCCATTCTGTCA-3' | 215 bp |
L014a 5'-CCAGTCAGGAGCACCTGTTC-3' | L014b 5'-AGAGCAGGGATGACTGTGGC-3' | 280 bp |
VIAS-OS4f 5'-CTCCTGGATGTTCTAGCAGT-3' | VIAS-OS4r 5'-CTCCTTGTCCAGCCATATAC-3' | 216–268 bp |
VIAS-OS14f 5'-CACTTCTCCGAATTTTAAAAGG-3' | VIAS-OS14r 5'-AGGAAGAGATGTGGAGTCCC-3' | 209–245 bp |