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Table 1 Sequences of primer pairs used in multiplex reactions and expected sizes of PCR products

From: Large scale sex typing of ostriches using DNA extracted from feathers

Forward primers Reverse primers Length of PCR product
SS1 5'-TCTACACCTAAGGAGTCCCATATT-3' SS2 5'-GGTCTACACCTGTTGAAAATCATT-3' 648 bp
OSM5f 5'-GTGGATCAGTTCAATCCTTGC-3' OSM5r 5'-GCCCAAGAAAATGATGGAGA-3' 232 bp
OSM7f 5'-AGCATACACATGCAGACCCC-3' OSM7r 5'-TGTTTCCTGCCATTCTGTCA-3' 215 bp
L014a 5'-CCAGTCAGGAGCACCTGTTC-3' L014b 5'-AGAGCAGGGATGACTGTGGC-3' 280 bp
VIAS-OS4f 5'-CTCCTGGATGTTCTAGCAGT-3' VIAS-OS4r 5'-CTCCTTGTCCAGCCATATAC-3' 216–268 bp
VIAS-OS14f 5'-CACTTCTCCGAATTTTAAAAGG-3' VIAS-OS14r 5'-AGGAAGAGATGTGGAGTCCC-3' 209–245 bp