Large-scale sex-typing analysis. A: The feather bulbs were submerged in the extraction solution contained on a 96 well PCR plate. B: After the extraction and neutralisation of the reactions, the template DNAs were transferred with a replicator to a novel 96 well PCR plate on which reaction mix had previously been placed. C: The plate was covered and PCR was then carried out. D: The PCR products were subjected to electrophoresis in an agarose gel to determine the animals' sex.