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Figure 2 | BMC Biotechnology

Figure 2

From: Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal

Figure 2

Dual non-invasive reporter visualization in embryos and adult mice. Transgenic animals are hemizygous for either the CK6/ECFP or YC5/EYFP transgenes. (a-d) blastocyst stage (E3.5) embryos, that are either fluorescent or non-fluorescent. Fluorescent embryos are either blue/cyan or yellow/green, thereby being hemizygous for either the CK6/ECFP or YC5/EYFP transgenes respectively. Non-fluorescent embryos (marked with an asterix on panel a) are of the wild type outbred ICR strain. (a) image taken under bright field with no epifluorescence. (b) dark field image taken through an EYFP filter. (c) dark field image taken through an ECFP filter. (d) dark field double exposure image acquired by consecutively using ECFP and EYFP filters. (e-h) two transgenic (TG/+) E13.5 embryos dissected free of their extraembryonic membranes, one is blue/cyan fluorescent (CK6/ECFP transgenic, left) and the other is yellow/green fluorescent (7YC5/EYFP transgenic, right). (e) image taken under bright field with no epifluorescence. (f) dark field image taken through an EYFP filter. (g), dark field image taken through an ECFP filter. (h) double exposure acquired by consecutively using ECFP and EYFP filters. (i-k) two three week old transgenic mice each exclusively expressing either the ECFP or EYFP reporter. All images shown are double exposures taken by consecutively using ECFP and then the EYFP filters under epifluorescence. (i) CK6/ECFP (TG/+) transgenic mouse bottom, YC5/EYFP (TG/+) transgenic mouse bottom. (j) CK6/ECFP (TG/+) transgenic mouse left, YC5/EYFP (TG/+) transgenic mouse right. (k) higher maginification view of the tails of the mice in i and j.

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