Table 3 Primers used in this study
From: Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase
Primer ID | Gene/element | Sequence (5′- 3′) | Reference |
|---|---|---|---|
b1 | bar | GCACCATCGTCAACCACTACATCG | [7] |
b2 | TCATGCCAGTTCCCGTGCTTG | [7] | |
h1 | hptII (pMBXS640) | TGATCGAAAAGTTCGACAGCGTCTC | This study |
h2 | CTCCAGTCAATGACCGCTGTTATGC | This study | |
h3 | hptII (pGPro8) | CTCCCGATTCCGGAAGTGCT | This study |
h4 | CATCGCCTCGCTCCAGTCAA | This study | |
r1 | bxb1 | CTGGTAGTCATCCGCCTGT | This study |
r2 | CTTCTAATTCCATCTGCGCCAC | This study | |
r3 | bxbNom | CAACTGGAGAGCTGCCAACAAC | This study |
r4 | ATATCCCAGCATGGCTTCGG | This study | |
g1 | gusAeGFP | AGCGCGTTACAAGAAAGC | This study |
g2 | AGAGATAACCTTCACCCGG | This study | |
g3 | GUSPlus | CGCAACCATATCGGATATGTCT | This study |
g4 | TGACATTCGGAATCTCCACG | This study | |
s1 | Stuffer | CTCGATCGGTTAGCATAGGCAG | This study |
s2 | GACTTAGCGTAGCAATGGCAACTG | This study | |
e1 | Stuffer construct | TGTAGTCTAGAGTGCTCCATTC | This study |
e2 | CGGCTTCAAATGGCGTATAGC | This study | |
e3 | Autoexcision | TCTAGAGTGCTCCATTCTCTCTCCTCG | This study |
e4 | GTTAAAACTGCCTGGCACAGCAATT | This study | |
c1 | Excised DNA fragment | GGGTTCCTATAGGGTTTCGCTCATG | This study |
c2 | TGATTTACCAGAAGCGGAGGAAGAA | This study | |
ActA | actin | CACTGGAATGGTCAAGGATG | [7] |
ActB | CTCCATGTCATCCCAGTTG | [7] |