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Table 1 Nucleotide sequences of the qPCR primers and probes used in the present study

From: Reduction in C-terminal amidated species of recombinant monoclonal antibodies by genetic modification of CHO cells

 

Forward primer

Reverse primer

Probe

PAM

GGCCGGATCCAATGTTTCAGAA

TCCCAAATGACGCATGTTTAATCTCT

CTGACACCAAAGAATTT

ACTB

AGCCACGCTCGGTCAG

CATCCTGCGTCTGGACCT

CCGGGACCTGACAGACT

GAPDH

CTGATTTTTCTTGCGTCGAGTTT

GAGTTGTGTTTGTGGACGAAGTAC

TCCGGTAAGACCTTTCG

EF1

CCTGGAATGGTGGTTACCTTTG

CATGGTGCATTTCAACAGACTTTACT

CCAGTCAACGTTACAACAGA

G6PD

ACAACATTGCCTGTGTGATCCT

CCCAAATTCATCAAAGTAGCCC

CCACGACCCTCAGTACCA

  1. PAM, peptidylglycine amidating monooxygenase; ACTB, β-actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EF1, elongation factor 1; G6PD, Glucose-6-phosphate dehydrogenase.
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BMC Biotechnology

ISSN: 1472-6750

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