| | FGF-1 content | ED50 (dilution) | AU/mL | U/mg |
|---|
S-FGF | 200 ng/mL | 41.5 | 50 | 2.5 × 105 |
T-SM | 10 ng/mL | 41.47 | 48 | 4.8 × 106 |
- Effective dose 50 (ED50) is determined by MTT assay curve using Balb/c 3 T3 cells. The dilution fold is used to determine that compounds are active at that concentration to proliferate 50% of the cell growth. The enzymatic activity of recombinant FGF-1 from transgenic S. miltiorrhiza (T-SM) is determined in terms of AU/mL in relation to a standard human acid FGF-1 (S-FGF) with a known activity (50 AU/mL). The specific enzymatic activity Units of FGF in T-SM was calculated as 48 AU/mL (41.47/41.50 × 50 AU/mL), with a conversion of (1000 × 1000/10 ng) × 48 = 4.8 × 106 U/mg.
