Figure 1

Plant expression construct pBI121- α FGF. The construct was made in the T-DNA vector, pBI121. The coding region of fgf-1 was fused with an alpha amylase signal (αSP). A Kozak sequence was inserted before the start codon. Pnos, promoter from nopaline synthase gene; nptII, neomycin phosphotransferase II gene; 3’nos, nopaline synthase terminator; p35S, CaMV 35S promoter. LB and RB, left and right border T-DNA sequence. Hind III, Pst I, Xho I and EcoR I were restriction enzymes used for cloning FGF-1.