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Table 1 Oligonucleotides used for the construction of the reporter vectors and EMSA

From: The transcription factor Ap-1 regulates monkey 20α-hydroxysteroid dehydrogenase promoter activity in CHO cells

Constructs Nucleotide sequence (5′ 3′)
pGL3 (-2005-Luc) GAG ACG GGG TTT CTC CAT
pGL3 (-890-Luc) TCT TAC AAG GCT AAT AAG AA
pGL3 (-513-Luc) AGT AAA CTT TAA TTT TTA AT
pGL3 (-273-Luc) AAA TAG AGA CTT GGA ATA GC
pGL3 (-306-Luc) ACA AAT CTC TTT GAA TAA TT
pGL3 (-288-Luc) TTT ATG TCT CAT AAA TAG AG
pGL3 (-70-Luc) CTG CTC ATT GGT CTT ATA GC
pGL3 Reverse CAT TTC TGT CAC TGG CCT
EMSA probe WT GAA TAA TTT ATG TCT CAT AAA TAG AGA CTT
EMSA probe mt-1 GAA TAA TTT Aca caT CAT A AA TAG AGA CTT
EMSA probe mt-2 GAA TAA TTT ATG TCg agg AAA TAG AGA CTT
EMSA probe mt-3 GAA TAA TTT Aca cag agg AAA TAG AGA CTT