Table 1 Oligonucleotides used for the construction of the reporter vectors and EMSA
Constructs | Nucleotide sequence (5′ 3′) |
|---|---|
pGL3 (-2005-Luc) | GAG ACG GGG TTT CTC CAT |
pGL3 (-890-Luc) | TCT TAC AAG GCT AAT AAG AA |
pGL3 (-513-Luc) | AGT AAA CTT TAA TTT TTA AT |
pGL3 (-273-Luc) | AAA TAG AGA CTT GGA ATA GC |
pGL3 (-306-Luc) | ACA AAT CTC TTT GAA TAA TT |
pGL3 (-288-Luc) | TTT ATG TCT CAT AAA TAG AG |
pGL3 (-70-Luc) | CTG CTC ATT GGT CTT ATA GC |
pGL3 Reverse | CAT TTC TGT CAC TGG CCT |
EMSA probe WT | GAA TAA TTT ATG TCT CAT AAA TAG AGA CTT |
EMSA probe mt-1 | GAA TAA TTT Aca caT CAT A AA TAG AGA CTT |
EMSA probe mt-2 | GAA TAA TTT ATG TCg agg AAA TAG AGA CTT |
EMSA probe mt-3 | GAA TAA TTT Aca cag agg AAA TAG AGA CTT |