Figure 9
From: Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B
Detection of 85 B multimers in α-85 B sandwich ELISA. 100 ng of capture scFv-Fc were coated to the wells. After blocking 100 ng of 85 B in PBS (untreated or boiled for 20 min at 100°C) were applied to the wells and incubated. Bound antigen was detected using A) MFU50-A10 B) MFU50-C10 and C) MFU50-D4-scFv-Fc-HRP at ~0.05 μg/mL and development with TMB. Negative control BSA A450 = 0.05. Multimer-detection is indicated by arrows.