Figure 2

Analysis of the epitope. A) Epitope mapping of α-85 B scFv-Fc with PepSpot membrane, B) Amino acid sequences of PepSpots and C) 3 D structure of 85 B and α-85 B antibody epitopes. A) Membrane was incubated with respectively 5 μg scFv-Fc, detection of bound scFv-Fc with goat α-human IgG(Fc)-HRP, development with TMB. Immunostained spots are circled. No cross reaction of the secondary antibody with the PepSpots was determined (data not shown). B) Amino acid sequences of peptides on 85 B PepSpot membrane. The immunostained spots and identified epitopes are highlighted. C) 3D structure of antigen 85 B, epitopes of α-85 B antibodies. Pdb1f0n (Resolution 1.9 Å, [36]) was modified with 3D molecule viewer (Invitrogen). Only protein chain A is shown, atoms are displayed as space filling balls, epitopes are marked in yellow, distance measurement in red.