Screening and genetic characterization of thermo-tolerant Synechocystis sp. PCC6803 strains created by adaptive evolution

Table 3 Mutations identified in mutIV-mix-2 in genes also identified in the monoclonal strains (Table 2 )

Position	Mutation type	Gene	WT	Mutant	Percentage of reads
98129*	AA change	pnp	aTg	aCg	100
194955	Frameshift	slr1098	-	T	11.70
195111	AA change	slr1098	Agt	Cgt	23.7
1103495*	AA change	pyk2	ttC	ttA	0.68
1103586*	AA change	pyk2	gGa	gAa	94.4
1133812*	Intergenic	Intergenic	G	T	51.4
1435867	Frameshift	cya1	ATGGTCGACG	-	41.3
2260225*	Intergenic	Intergenic	-	A	100
2466798*	AA change	clpC	gTg	gCg	45.5
2466808*	AA change	clpC	Cgt	Tgt	8.16
2466882	AA change	clpC	gTa	gCa	10
2467723*	AA change	clpC	Gac	Tac	4.65
2468031*	AA change	clpC	gTt	gCt	20.5
2579430	Frameshift	sll0064	-	CCCTG	61
2717197	Frameshift	pyrR	AAGGTTAA	GTGGCTTTAA…	10.9
2717187	Frameshift	pyrR	-	TTAACTCCACTAA	70.4

Positions marked with a *correspond to identical mutation positions for monoclonal strains.

ISSN: 1472-6750