Figure 2From: Expression, purification, and characterization of rhTyrRSPurification of rhTyrRS using a three-step procedure. M, standard protein marker (20–250 kD). Lane 1, soluble lysate from induced cells; Lane 2, cation exchange chromatography wash fraction; Lane 3, gel filtration wash fraction; Lane 4, anion exchange chromatography wash fraction; Lane 5, western blot assay of rhTyrRS.Back to article page