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Table 4 Combinations of GenSolve, Qiagen and NucleoSpin protocols for DNA extraction from DBS: GQ versus NN and Nn methods

From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling

       65°C, 65 min   
  Sample code DNA (ng/μl) DNA (ng) 260/280 260/230 260/280 260/230 Protocol
  NCS 1a 25.3 1062 1.84 1.15 1.88 1.18 GQ
NCS 1b 40.0 1802 1.82 1.84 1.90 1.90 NN
NCS 2a 28.8 1209 1.87 1.27 1.89 1.33 GQ
NCS 2b 47.3 2127 1.89 2.00 1.90 1.91 NN
NCS 3a 15.2 638 2.02 1.09 1.93 1.15 GQ
NCS 3b 27.6 1271 1.82 1.59 1.91 1.61 NN
NCS 4-4a 23.0 987 1.93 0.93 1.96 1.03 GQ
NCS 4-4b 30.0 1348 1.85 1.72 1.90 1.81 NN
NCS 4-5a 33.4 1435 1.82 1.01 1.83 1.02 GQ
NCS 4-5b 35.4 1593 1.98 1.94 1.87 1.76 NN
NCS 4-6a 19.9 856 2.01 1.14 1.97 1.10 GQ
NCS 4-6b 33.9 1525 1.93 1.85 1.89 1.82 NN
Precipitation buffer in NN changed to ethanol, leading to protocol Nn NCS 4-7a 18.0 774 1.76 0.87 1.86 0.84 GQ
NCS 4-7b 25.7 1155 1.93 1.37 1.81 1.35 Nn
NCS 4-8a 14.7 617 1.99 0.90 1.94 0.91 GQ
NCS 4-8b 24.6 1105 1.92 1.33 1.85 1.42 Nn
NCS 4-9a 26.1 1121 1.90 1.20 1.94 1.26 GQ
NCS 4-9b 39.0 1754 1.95 1.89 1.88 1.77 Nn
  1. Two punches, each having 9 mm diameter, were analyzed per DBS. Punches labeled “a” were tested with GQ while their matched pairs, labeled “b”, were tested with NN. When the DNA precipitation buffer in NN was changed to ethanol, the resultant protocol was termed Nn. Average eluate volumes by GQ and NN/Nn were 42 μl and 45 μl, respectively. Data represent averages of 2–4 readings per sample.