Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling

BMC Biotechnology

Table 4 Combinations of GenSolve, Qiagen and NucleoSpin protocols for DNA extraction from DBS: GQ versus NN and Nn methods

						65°C, 65 min
	Sample code	DNA (ng/μl)	DNA (ng)	260/280	260/230	260/280	260/230	Protocol
	NCS 1a	25.3	1062	1.84	1.15	1.88	1.18	GQ
	NCS 1b	40.0	1802	1.82	1.84	1.90	1.90	NN
	NCS 2a	28.8	1209	1.87	1.27	1.89	1.33	GQ
	NCS 2b	47.3	2127	1.89	2.00	1.90	1.91	NN
	NCS 3a	15.2	638	2.02	1.09	1.93	1.15	GQ
	NCS 3b	27.6	1271	1.82	1.59	1.91	1.61	NN
	NCS 4-4a	23.0	987	1.93	0.93	1.96	1.03	GQ
	NCS 4-4b	30.0	1348	1.85	1.72	1.90	1.81	NN
	NCS 4-5a	33.4	1435	1.82	1.01	1.83	1.02	GQ
	NCS 4-5b	35.4	1593	1.98	1.94	1.87	1.76	NN
	NCS 4-6a	19.9	856	2.01	1.14	1.97	1.10	GQ
	NCS 4-6b	33.9	1525	1.93	1.85	1.89	1.82	NN
Precipitation buffer in NN changed to ethanol, leading to protocol Nn	NCS 4-7a	18.0	774	1.76	0.87	1.86	0.84	GQ
	NCS 4-7b	25.7	1155	1.93	1.37	1.81	1.35	Nn
	NCS 4-8a	14.7	617	1.99	0.90	1.94	0.91	GQ
	NCS 4-8b	24.6	1105	1.92	1.33	1.85	1.42	Nn
	NCS 4-9a	26.1	1121	1.90	1.20	1.94	1.26	GQ
	NCS 4-9b	39.0	1754	1.95	1.89	1.88	1.77	Nn

Two punches, each having 9 mm diameter, were analyzed per DBS. Punches labeled “a” were tested with GQ while their matched pairs, labeled “b”, were tested with NN. When the DNA precipitation buffer in NN was changed to ethanol, the resultant protocol was termed Nn. Average eluate volumes by GQ and NN/Nn were 42 μl and 45 μl, respectively. Data represent averages of 2–4 readings per sample.

ISSN: 1472-6750