Development of late blight resistant potatoes by cisgene stacking

BMC Biotechnology

Table 2 Marker-free transformation of two R genes ( Rpi-vnt1.1:Rpi-sto1 ) to different potato varieties; Marker-free transformation frequencies

Variety	explants #	shoots #	PCR + #	frequency %	bbf #	bbf %
Atlantic	200	497	0/0/12	2.4	9	75
Bintje	200	590	2/0/6	1.0	5	83
Potae9	200	428	0/0/7	1.6	5	71
total	600	1515	2/0/25	1.7	19	76

#explants; number of explants; #shoots: number of shoots tested; #PCR+: the number of shoots containing Rpi-sto1, Rpi-vnt1.1, or both genes, respectively, as detected by PCR; % frequency: transformation frequency, as percentage of PCR + shoots, carrying both Rpi-sto1 and Rpi-vnt1.1, over the number of tested shoots; #bbf: number of vector backbone free events; % bb: percentage of vector backbone containing PCR + shoots, carrying both Rpi-sto1 and Rpi-vnt1.1.

ISSN: 1472-6750