Skip to main content

Table 2 Marker-free transformation of two R genes ( Rpi-vnt1.1:Rpi-sto1 ) to different potato varieties; Marker-free transformation frequencies

From: Development of late blight resistant potatoes by cisgene stacking

Variety explants # shoots # PCR + # frequency % bbf # bbf %
Atlantic 200 497 0/0/12 2.4 9 75
Bintje 200 590 2/0/6 1.0 5 83
Potae9 200 428 0/0/7 1.6 5 71
total 600 1515 2/0/25 1.7 19 76
  1. #explants; number of explants; #shoots: number of shoots tested; #PCR+: the number of shoots containing Rpi-sto1, Rpi-vnt1.1, or both genes, respectively, as detected by PCR; % frequency: transformation frequency, as percentage of PCR + shoots, carrying both Rpi-sto1 and Rpi-vnt1.1, over the number of tested shoots; #bbf: number of vector backbone free events; % bb: percentage of vector backbone containing PCR + shoots, carrying both Rpi-sto1 and Rpi-vnt1.1.