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Figure 5 | BMC Biotechnology

Figure 5

From: Ubiquitin-like prokaryotic MoaD as a fusion tag for expression of heterologous proteins in Escherichia coli

Figure 5

Expression of insulin chains with MoaD fusion. Insulin A chain (upper panel) or B chain (lower panel) fused with MoaD at their N-termini, were expressed in E. coli BL21 (DE3) pLysS. ThiS-fused chains were used as control. Total cell lysate from uninduced (−) or induced (+) cells with IPTG, and the soluble (S) or insoluble fraction (I) of induced cells were electrophoresed on 15% SDS-PAGE, shown in left panel. Expressed proteins were verified by Western blot probed with anti His-tag antibody, shown in right panel. Arrows highlight expressed proteins at expected positions.

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BMC Biotechnology

ISSN: 1472-6750

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