Figure 1

Enhanced expression of EGFP fused with MoaD. (A) The recombinant EGFP proteins without fusion (EGFP), and with MoaD-tag fused at N-terminus (left panels) or C-terminus (right panels), were induced by 1 mM IPTG for 4 h at 37°C. ThiS-tag fusions were used as positive control. Total cell lysates were resolved on 12% SDS-PAGE (upper panels) and analyzed by immunoblot with anti His-tag antibody (lower panels). (B) At 37°C induced by 1 mM IPTG or (C) at room temperature induced by 0.1 mM IPTG, cell growth (black lines) was recorded by measuring absorbance at 600 nm, and the fluorescence of cells was measured (excitation 488 nm; emission 509 nm) and normalized to corresponding OD₆₀₀ (blue lines). Triangle is for EGFP, open circle for ThiS fusion, and solid circle for MoaD fusion. Each point represents mean and SD of 4 independent experiments. *p < 0.05; **p < 0.01 comparing to EGFP control.