Figure 5

Kinetic plot obtained with recombinant CgChoA from C. gleum. The specific activity (U/mg) was plotted versus the substrate concentration. An enzyme concentration of 3.57 mg/L was used and the cholesterol stock solution/dispersion was prepared and serially diluted in (●) water only, (▲) water and taurocholate, (♦) water containing 5% Triton X-100 and taurocholate, or in (■) water containing 5% (v/v) Triton X-100.