A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Table 4 Repeatability of the quadruplex real-time PCR method for GM cotton GHB119, T304-40 and Cry2Ae detection

Amount of DNA (copies/reaction)	Ct value					SD^r*	RSD^r(%)	SD^R*	RSD^R(%)
Amount of DNA (copies/reaction)	REP1	REP2	REP3	REP4	Mean	SD^r*	RSD^r(%)	SD^R*	RSD^R(%)
GHB119
11600	24.39	24.51	24.57	24.40	24.47	0.09	0.36	0.05	0.19
2320	26.80	26.83	26.96	26.78	26.84	0.08	0.30	0.03	0.12
464	29.23	29.28	29.46	29.49	29.37	0.13	0.43	0.03	0.11
232	30.26	30.41	30.50	30.35	30.38	0.10	0.34	0.06	0.19
92.8	31.69	31.72	31.83	31.69	31.73	0.07	0.22	0.08	0.25
46.4	32.53	32.89	32.93	32.69	32.76	0.19	0.57	0.11	0.33
T304-40
11600	24.68	24.70	24.72	24.69	24.70	0.02	0.07	0.05	0.20
2320	27.04	27.07	27.10	27.23	27.11	0.08	0.30	0.02	0.08
464	29.67	29.68	29.70	29.84	29.72	0.08	0.26	0.01	0.02
232	30.72	30.77	30.89	30.96	30.83	0.11	0.36	0.05	0.17
92.8	31.96	32.05	32.16	31.78	31.99	0.16	0.50	0.10	0.30
46.4	33.08	33.25	33.36	33.43	33.28	0.15	0.46	0.15	0.46
Cry2Ae
11600	23.29	23.32	23.34	23.21	23.29	0.06	0.24	0.04	0.17
2320	25.64	25.68	25.71	25.73	25.69	0.04	0.15	0.04	0.17
464	28.03	28.15	28.17	28.22	28.14	0.08	0.28	0.08	0.27
232	29.02	29.19	29.20	29.24	29.16	0.10	0.33	0.05	0.17
92.8	30.63	30.68	30.83	30.85	30.75	0.11	0.36	0.07	0.23
46.4	31.99	32.02	32.05	32.14	32.05	0.06	0.20	0.10	0.31

*SD^r: standard deviation of four parallel reactions; SD^R: standard deviation of three experiments; RSD^r: relative standard deviation of four parallel reactions; RSD^R: relative standard deviation of three experiment.

ISSN: 1472-6750