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Table 4 Repeatability of the quadruplex real-time PCR method for GM cotton GHB119, T304-40 and Cry2Ae detection

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Amount of DNA (copies/reaction) Ct value SDr* RSDr(%) SDR* RSDR(%)
REP1 REP2 REP3 REP4 Mean
GHB119
11600 24.39 24.51 24.57 24.40 24.47 0.09 0.36 0.05 0.19
2320 26.80 26.83 26.96 26.78 26.84 0.08 0.30 0.03 0.12
464 29.23 29.28 29.46 29.49 29.37 0.13 0.43 0.03 0.11
232 30.26 30.41 30.50 30.35 30.38 0.10 0.34 0.06 0.19
92.8 31.69 31.72 31.83 31.69 31.73 0.07 0.22 0.08 0.25
46.4 32.53 32.89 32.93 32.69 32.76 0.19 0.57 0.11 0.33
T304-40
11600 24.68 24.70 24.72 24.69 24.70 0.02 0.07 0.05 0.20
2320 27.04 27.07 27.10 27.23 27.11 0.08 0.30 0.02 0.08
464 29.67 29.68 29.70 29.84 29.72 0.08 0.26 0.01 0.02
232 30.72 30.77 30.89 30.96 30.83 0.11 0.36 0.05 0.17
92.8 31.96 32.05 32.16 31.78 31.99 0.16 0.50 0.10 0.30
46.4 33.08 33.25 33.36 33.43 33.28 0.15 0.46 0.15 0.46
Cry2Ae
11600 23.29 23.32 23.34 23.21 23.29 0.06 0.24 0.04 0.17
2320 25.64 25.68 25.71 25.73 25.69 0.04 0.15 0.04 0.17
464 28.03 28.15 28.17 28.22 28.14 0.08 0.28 0.08 0.27
232 29.02 29.19 29.20 29.24 29.16 0.10 0.33 0.05 0.17
92.8 30.63 30.68 30.83 30.85 30.75 0.11 0.36 0.07 0.23
46.4 31.99 32.02 32.05 32.14 32.05 0.06 0.20 0.10 0.31
  1. *SDr: standard deviation of four parallel reactions; SDR: standard deviation of three experiments; RSDr: relative standard deviation of four parallel reactions; RSDR: relative standard deviation of three experiment.
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